Complete Product Description

The ABI 394 (4 or 2 columns) synthesizer is an economical- versatile- medium-throughput platform that synthesizes DNA oligonucleotides with fast cycle times- low reagent consumption. This system is ideal for synthesizing primers of various lengths as well as complex modified oligonucleotides plus dye-labeled primers and probes. It has the flexibility to synthesize sequences with lengths greater than 50-mer at scales of 40 nmol- 0.2 umol- and 1 umol.Each instrument includes trityl monitoring as standard. An optional Apple PC can be added to enable easier cycle editing and network capabilities.

Oligonucleotide Synthesis with the Applied Biosystems model 394 DNA Synthesizers can extend two or four oligonucleotides simultaneously at a rate of 12 bases per hour. Synthesis occurs from the 3? towards the 5? end of the oligonucleotide on pre-packed- pre-derivatised- solid phase columns.

Oligonucleotides of 80 bases can be easily achieved by the solid phase synthesis methodology- as there are no secondary structure effects to inhibit chain elongation. It is feasible to make much longer polymers - but in practice- as the chain extends beyond 80 bases- there is an increased probability that bases near the 3? end may become chemically modified.

DNA Quality with THe ABI 394

Expected performance: you should be able to consistently synthesize oligonucleotides with average coupling yields greater than 98%. For PCR or sequencing work- the oligos we provide require no further purification

Modifications

A wide range of unusual or modified bases and labels can be incorporated into an oligonucleotide during synthesis. Typical modifications include: Amino Link -Biotin -Flourescein (6-FAM/ HEX/ TET) -Inosine and Phosphorylation

Applied Biosystems Model 394 Specifications