Complete Product Description

The LabAlliance Ultra Fast Flow System is Technology to truely Change the Proteome Paradigm.

The Ultra High Pressure (UHP) Liquid Chromatography System provides reproducible high-speed separations of intact proteins in minutes. This technology- when coupled with existing methods- allows a new approach to Proteomics. Imagine high resolutions native protein separations in under 10 minutes- with resolving power that would normally require one hour. In addition- the system facilitates loading that allows multiple paths of investigation on the collected protein fractions- and the ability to run multidimensional separations with orthogonal feed back to mine information never before available.

Orthogonal Confirmation of Proteins

The use of a top down approach and the traditional MudPIT approach allows confirmation of a hit through MudPIT identification with a native protein MW. If the protein is not found in the fraction- the possibility of post translational changes or other modifications can be explored with secondary separations and MS/MS techniques to identify the proteins in the suspect fraction.

Increased Sensitivity of Low Abundance Proteins

The use of the top down fractionation of proteins will dramatically increase the sensitivity of low abundance proteins as shown in the following figure. The abundant proteins will no longer mask the low abundance proteins. The column loading can be calculated to allow extremely low abundance proteins to be seen in the MudPIT or MS/ MS scans of the native protein.

Resolution of Post Translational Changes

The use of a secondary separation on a fraction will allow for further definition of what is occurring in the proteome by allowing post translational modifications to be identified by either MS/MS or further chromatography separation.

Faster Proteome Library Generation

Native protein separations in minutes with the ability to collect fractions and use multidimensional and orthogonal means of protein identification will allow libraries to be developed in a faster manner than a single approach. Leave the MudPIT and see the light of Topdown and Bottom-up Proteomics.

UFFLC of the Proteome Ultra High Pressure and Ultra Fast Flow Liquid Chromatography

EZChrom SI Specifications What is EZChrom SI? EZChrom SI is a complete data acquisition- processing- and control package for 1 instrument. Based on the highly popular EZChrom EliteTM software package- EZChrom SI is the ideal solution for laboratories who want a simple-to-use chromatography data system with comprehensive software features and instrument control. It is intended to provide the basic requirements for data acquisition and optional instrument control for a single workstation. EZChrom SI consists of two software modules: EZChrom SI Configuration and EZChrom SI. With EZChrom SI- instrument control and analysis is reduced to just 3 steps: Configure- Run- and Report. Once the instrument is configured- any user can launch EZChrom SI- immediately choose a method and submit samples for acquisition. All this operation is done in EZChrom SI?s Single Window Access environment - a design that greatly simplifies tasks for the casual or novice user. Either single runs or sequence runs can be performed. Users can still take advantage of the intelligent features in SmartSequenceTM- a powerful tool that a powerful tool that automates much of the decision making process based on user defined criteria. If methods or sequences need to be created from scratch- the simple wizards will guide you through a stepwise process.

Flexible report management

Within any method exists a report template. Users simply select from standard templates and can produce the reports they require. EZChrom SI?s custom report editor may be used to modify existing templates or to create new report templates.

Ultra Fast Flow Chromatogrpahy System

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